- Cell line development and Analysis
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- Overview
Cell Line Development and Analysis
At Mano-Tri Pharma, our Molecular Biology Services include advanced cell line development and analysis, supporting applications in biotherapeutic discovery, assay development, and reagent production. With deep technical expertise and a state-of-the-art Molecular Biology Laboratory, we generate high-yield, homogenous, and stable clones across diverse cell types, including hard-to-transfect and primary cells.
Cell Line Development
We design and produce recombinant cell lines for consistent and high-level expression of:
- Tagged and untagged proteins
- GPCRs, ion channels, and receptor, ligand complexes
- Transcription factors, microRNA targets, and reporter genes
Cell Culture Capacity
- Adherent cells: From 96-well plates to multilayer cell stacks
- Suspension systems: 30 mL to 3 L spinners
- Scale-up: 2–10 L lab scale, 1–25 L wave bioreactors, and 50–300 L pilot scale for GMP pharmaceutical manufacturing readiness
Cell Line Characterization
Our bioanalytical laboratory services offer precise, multi-platform analysis through:
- Real-Time PCR (96/384 format), Northern & Western blotting
- Flow Cytometry (FACS) and functional cell-based assays
- Immunoassays, Immunofluorescence, and Immunocytochemistry
Cell Banking Services
To ensure reliability and continuity in research and production, we provide:
- Expansion and cryopreservation of research cell lines
- Viability testing and Research Cell Bank (RCB) preparation
- Comprehensive sterility and Mycoplasma testing for quality assurance
- Why Partner With Us
Expertise Rooted in Science
State-of-the-Art Molecular Biology Laboratory
Unmatched Quality & Analytical Excellence
Strong Focus on Cell Line Stability & Performance
Frequently Asked Quesions
Our Molecular Biology Services combine recombinant DNA design, transfection optimization, stability analysis, and multi-platform validation to provide high-yield, stable cell lines. We provide precise characterization using modern Bioanalytical Laboratory Services, which include qPCR, flow cytometry, blotting methods, and functional tests.
Yes. Our cell culture infrastructure, from 96-well plates to 300 L pilot scale: enables a smooth transition to GMP pharmaceutical manufacturing. This enables modified cell lines to be scaled for biotherapeutics, assay development, biosimilar manufacture, and upstream operations in bigger CDMO pipelines.
Absolutely. Mano-Tri provides Analytical Method Development Services to validate gene expression, establish protein productivity, maintain clone stability, and assist regulatory paperwork. These analytical insights are critical for the biologics and biosimilar manufacturing workflows.
Our stable and high-expression cell lines serve as the foundation for downstream programs such as monoclonal antibody development services, recombinant protein synthesis, assay reagent development, and early-phase bioassay design. We also work smoothly with CDMO partners on peptide programs including Solid Phase Peptide Synthesis and other medicinal platforms.
- Yes. Our designed cell lines and molecular analytics meet the needs of biosimilar product manufacturers in India, top biosimilar companies in India, and developers working with Microbial Fermentation Services or Microbial Fermentation Products. We provide robust upstream characterisation to support fermentation, biologics manufacture, and regulatory-grade biosimilar development.
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