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- Overview
Molecular Biology
Recombinant DNA Engineering
We specialize in advanced gene cloning and vector construction using techniques such as:
- High-throughput recombination-based cloning (Gateway®)
- De novo cloning via PCR/RT-PCR/homology-based methods
- Synthetic gene and oligo-linker cloning
- Custom vector construction for specific applications
Mutagenesis
We design site-directed and random mutagenesis strategies, including:
- Point mutations, insertions, deletions, or replacements
- SOE PCR, megaprimer, and DpnI-based methods
Genome Walking
Gene Library Construction & Screening
- Construction of cDNA, BAC, cosmid, and phage libraries
- Screening through radioactive hybridization or functional assays
Strain Engineering
We develop optimized bacterial and yeast strains for enhanced recombinant protein production using:
- Gene knock-out, insertional inactivation, and site-directed modification
Gene Expression Analysis
Our bioanalytical laboratory services enable accurate transcriptional and translational studies through:
- Real-Time PCR, Northern and Western blotting
- ELISA, Flow Cytometry, Mass Spectrometry, and Reporter assays
Oligonucleotide Synthesis Capabilities
Expanding our molecular biology scope, we also offer custom oligonucleotide synthesis and RNA oligonucleotide synthesis, enabling targeted gene modulation, expression profiling, and diagnostic assay development.
- Why Partner With Us
Integrated Molecular Biology & Bioanalytics
High-Precision Genetic Engineering
Expertise Across Complex Biological Systems
Custom Oligonucleotide Synthesis Excellence
Scalable, Research-to-Industry Approach
Frequently Asked Quesions
Mano-Tri Pharma provides comprehensive molecular biology services such as recombinant DNA engineering, mutagenesis, genome walking, and gene expression profiling. With the help of advanced Bioanalytical Laboratory Services, we provide precise, scalable, and development-ready data for research, diagnostics, and biomanufacturing initiatives.
Yes. We specialize in custom oligonucleotide synthesis and therapeutic-grade oligonucleotide synthesis, which allows for gene regulation, expression profiling, cloning procedures, and assay development. These capabilities enable both molecular biology research and GMP-driven development workflows.
Absolutely. Our strain engineering skills are seamlessly integrated with upstream Microbial Fermentation Services, which include recombinant protein production optimization, pathway modification, and increased expression platforms. We also help to scale up initiatives that combine bacterial fermentation processes and microbial fermentation manufacturing.
Yes. Mano-Tri offers Analytical Method Development Services to validate constructs, measure expression, and ensure genetic accuracy through qPCR, sequencing, blotting, and mass-spec profiling. These methods guarantee regulatory-ready documentation for GMP, biologics, and biosimilar research.
Our genetic engineering, strain generation, and expression analysis methods are completely compatible with GMP-compliant manufacturing and CDMO operations. This establishes Mano-Tri as a powerful partner for CDMO companies in India, allowing for a seamless transition from molecular engineering to large-scale biologics, biosimilars, peptide projects, and next-generation medicinal development.
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